IGTR Panel v1.0

Catalog Number:

#1001941
#1001942

IGTR Panel v1.0 covers the entire Immunoglobulin (IG) and T cell receptor (TR) coding gene segments of the human genome, which can be used forthe enrichment of segments before or after rearranged by V(D)JCrecombination at the DNA level, and the enrichment of mature transcript at the RNA level.

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Performance

DNA Capture Performance

Figure 1. Capture performance of IGTR Panel v1.0 in different DNA libraries.Libraries were prepared using NadPrep DNA Library Preparation Kit (for Illumina®) coupled with NadPrep UDI Adapter Kit (for Illumina®). IGTR Panel v1.0 were respectively used to complete hybridization capture, and sequenced on Illumina Novaseq 6000 with PE150. The BWA was used for alignment of raw reads to the reference genome (hg38). A. Mappability and On-target rate; B. Targeted covered;
Note：The DNA_1-3 samples were: Ramos cell line DNA; Jurkat cell line DNA; Human Gnomic DNA (Promega-male, G1471).

RNA Capture Performance

Figure 2. Capture performance of IGTR Panel v1.0 in different RNA libraries. Libraries were prepared using NadPrep Total RNA-To-DNA Module coupled with NadPrep DNA Library Preparation Kit (for Illumina®). IGTR Panel v1.0 were respectively used to complete hybridization capture, and sequenced on Illumina Novaseq 6000 with PE150. Analysis was performed using salmon.
Note：The RNA_1-3 samples were: Ramos cell line total RNA; Jurkat cell line total RNA; Human Blood Peripheral Leukocytes Total RNA (Clontech, 636592).

Example Coverage of Transcript by RNACap

Ramos Cell Line

Jurkat Cell Line

Figure 3. Example coverage of transcripts captured by RNA from different cell lines with IGTR Panel v1.0.

Sensitivity Performance

Figure 4. Capture sensitivity performance of IGTR Panel v1.0 in both DNA and RNA libraries. Libraries were prepared using NadPrep Total RNA-To-DNA Module coupled with NadPrep DNA Library Preparation Kit (for Illumina®). IGTR Panel v1.0 were respectively used to complete hybridization capture, and sequenced on Illumina Novaseq 6000 with PE150.
Note：Ramos+Jurkat DNA were serially diluted with Human Genomic DNA (Promega-male, G1471); Ramos+Jurkat RNA were serially diluted with Human Blood Peripheral Leukocytes Total RNA (Clontech, 636592).

Gene List

IG Coding Gene

IGHV1-2	IGHV1-3	IGHV1-18	IGHV1-24	IGHV1-45	IGHV1-46	IGHV1-58	IGHV1-69	IGHV1-69-2	IGHV1-69D
IGHV1OR15-1	IGHV1OR21-1	IGHV1OR16-2	IGHV1OR15-9	IGHV2-5	IGHV2-26	IGHV2-70	IGHV2-70D	IGHV2OR16-5	IGHV3-7
IGHV3-11	IGHV3-13	IGHV3-15	IGHV3-16	IGHV3-20	IGHV3-21	IGHV3-23	IGHV3-25	IGHV3-30	IGHV3-33
IGHV3-35	IGHV3-38	IGHV3-43	IGHV3-48	IGHV3-49	IGHV3-53	IGHV3-62	IGHV3-64	IGHV3-66	IGHV3-72
IGHV3-73	IGHV3-74	IGHV3-64D	IGHV3OR16-6	IGHV3OR15-7	IGHV3OR16-8	IGHV3OR16-9	IGHV3OR16-10	IGHV3OR16-12	IGHV3OR16-13
IGHV3OR16-17	IGHV4-4	IGHV4-28	IGHV4-31	IGHV4-34	IGHV4-39	IGHV4-59	IGHV4-61	IGHV4OR15-8	IGHV5-51
IGHV5-10-1	IGHV6-1	IGHV7-81	IGHV7-4-1	IGHV8-51-1	IGHD1-1	IGHD1-7	IGHD1-14	IGHD1-20	IGHD1-26
IGHD1OR15-1A	IGHD1OR15-1B	IGHD2-2	IGHD2-8	IGHD2-15	IGHD2-21	IGHD2OR15-2A	IGHD2OR15-2B	IGHD3-3	IGHD3-9
IGHD3-10	IGHD3-16	IGHD3-22	IGHD3OR15-3A	IGHD3OR15-3B	IGHD4-4	IGHD4-11	IGHD4-17	IGHD4-23	IGHD4OR15-4A
IGHD4OR15-4B	IGHD5-5	IGHD5-12	IGHD5-18	IGHD5-24	IGHD5OR15-5A	IGHD5OR15-5B	IGHD6-6	IGHD6-13	IGHD6-19
IGHD6-25	IGHD7-27	IGHJ1	IGHJ2	IGHJ3	IGHJ4	IGHJ5	IGHJ6	IGHA1	IGHA2
IGHD	IGHE	IGHG1	IGHG2	IGHG3	IGHG4	IGHGP	IGHM	IGKV1-5	IGKV1-6
IGKV1-8	IGKV1D-8	IGKV1-9	IGKV1-12	IGKV1D-12	IGKV1-13	IGKV1D-13	IGKV1-16	IGKV1D-16	IGKV1-17
IGKV1D-17	IGKV1-27	IGKV1-33	IGKV1D-33	IGKV1-37	IGKV1D-37	IGKV1-39	IGKV1D-39	IGKV1D-42	IGKV1D-43
IGKV1OR2-108	IGKV2-24	IGKV2D-24	IGKV2D-26	IGKV2-28	IGKV2D-28	IGKV2-29	IGKV2D-29	IGKV2-30	IGKV2D-30
IGKV2-40	IGKV2D-40	IGKV3-7	IGKV3D-7	IGKV3-11	IGKV3D-11	IGKV3-15	IGKV3D-15	IGKV3-20	IGKV3D-20
IGKV3OR2-268	IGKV4-1	IGKV5-2	IGKV6-21	IGKV6D-21	IGKV6D-41	IGKJ1	IGKJ2	IGKJ3	IGKJ4
IGKJ5	IGKC	IGLV1-36	IGLV1-40	IGLV1-41	IGLV1-44	IGLV1-47	IGLV1-50	IGLV1-51	IGLV2-8
IGLV2-11	IGLV2-14	IGLV2-18	IGLV2-23	IGLV2-33	IGLV3-1	IGLV3-9	IGLV3-10	IGLV3-12	IGLV3-16
IGLV3-19	IGLV3-21	IGLV3-22	IGLV3-25	IGLV3-27	IGLV3-32	IGLV4-3	IGLV4-60	IGLV4-69	IGLV5-37
IGLV5-45	IGLV5-48	IGLV5-52	IGLV6-57	IGLV7-43	IGLV7-46	IGLV8-61	IGLV8OR8-1	IGLV9-49	IGLV10-54
IGLV11-55	IGLJ1	IGLJ2	IGLJ3	IGLJ4	IGLJ5	IGLJ6	IGLJ7	IGLC1	IGLC2
IGLC3	IGLC6	IGLC7	IGLL1	IGLL5

TR Coding Gene

TRAV1-1	TRAV1-2	TRAV2	TRAV3	TRAV4	TRAV5	TRAV6	TRAV7	TRAV8-1	TRAV8-2
TRAV8-3	TRAV8-4	TRAV8-6	TRAV8-7	TRAV9-1	TRAV9-2	TRAV10	TRAV12-1	TRAV12-2	TRAV12-3
TRAV13-1	TRAV13-2	TRAV14DV4	TRAV16	TRAV17	TRAV18	TRAV19	TRAV20	TRAV21	TRAV22
TRAV23DV6	TRAV24	TRAV25	TRAV26-1	TRAV26-2	TRAV27	TRAV29DV5	TRAV30	TRAV34	TRAV35
TRAV36DV7	TRAV38-1	TRAV38-2DV8	TRAV39	TRAV40	TRAV41	TRAJ1	TRAJ2	TRAJ3	TRAJ4
TRAJ5	TRAJ6	TRAJ7	TRAJ8	TRAJ9	TRAJ10	TRAJ11	TRAJ12	TRAJ13	TRAJ14
TRAJ15	TRAJ16	TRAJ17	TRAJ18	TRAJ19	TRAJ20	TRAJ21	TRAJ22	TRAJ23	TRAJ24
TRAJ25	TRAJ26	TRAJ27	TRAJ28	TRAJ29	TRAJ30	TRAJ31	TRAJ32	TRAJ33	TRAJ34
TRAJ35	TRAJ36	TRAJ37	TRAJ38	TRAJ39	TRAJ40	TRAJ41	TRAJ42	TRAJ43	TRAJ44
TRAJ45	TRAJ46	TRAJ47	TRAJ48	TRAJ49	TRAJ50	TRAJ52	TRAJ53	TRAJ54	TRAJ56
TRAJ57	TRAJ58	TRAJ59	TRAJ61	TRAC	TRBV2	TRBV3-1	TRBV4-1	TRBV4-2	TRBV5-1
TRBV5-3	TRBV5-4	TRBV5-5	TRBV5-6	TRBV5-7	TRBV6-1	TRBV6-2	TRBV6-4	TRBV6-5	TRBV6-6
TRBV6-7	TRBV6-8	TRBV7-1	TRBV7-2	TRBV7-3	TRBV7-4	TRBV7-6	TRBV7-7	TRBV7-9	TRBV9
TRBV10-1	TRBV10-2	TRBV10-3	TRBV11-1	TRBV11-2	TRBV11-3	TRBV12-3	TRBV12-4	TRBV12-5	TRBV13
TRBV14	TRBV15	TRBV16	TRBV17	TRBV18	TRBV19	TRBV20-1	TRBV20OR9-2	TRBV23-1	TRBV23OR9-2
TRBV24-1	TRBV24OR9-2	TRBV25-1	TRBV27	TRBV28	TRBV29-1	TRBV29OR9-2	TRBV30	TRBD1	TRBD2
TRBJ1-1	TRBJ1-2	TRBJ1-3	TRBJ1-4	TRBJ1-5	TRBJ1-6	TRBJ2-1	TRBJ2-2	TRBJ2-3	TRBJ2-4
TRBJ2-5	TRBJ2-6	TRBJ2-7	TRBJ2-2P	TRBC1	TRBC2	TRGV1	TRGV2	TRGV3	TRGV4
TRGV5	TRGV8	TRGV9	TRGV10	TRGV11	TRGJ1	TRGJ2	TRGJP1	TRGJP2	TRGJP
TRGC1	TRGC2	TRDV1	TRDV2	TRDV3	TRDD1	TRDD2	TRDD3	TRDJ1	TRDJ2
TRDJ3	TRDJ4	TRDC

For research use only. Not for use in diagnostic procedures.

Catalog	Color of Tube	Product	Volume	Package/Storage temperature
1001941		IGTR Panel v1.0, 96 rxn	415 μL	–20℃
1001942		IGTR Panel v1.0, 16 rxn	70 μL	–20℃

Can RNA samples be directly used for library preparation with this kit?

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

What is the recommended input range? How to handle inputs exceeding this range?

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

What is the insert size of this kit? How to select sequencing read length?

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

What is the recommended sequencing data volume for IGTR immune repertoire analysis?

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Is this kit suitable for minimal residual disease (MRD) monitoring?

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.