HiSNP-series Panel

Catalog Number:

#1001831 (HiSNP Panel v1.0, 96 rxn)
#1001838 (HiSNP Ultra Panel v1.0, 960 rxn)
#1001839 (HiSNP Ultra Panel v1.0, 1920 rxn)

HiSNP Ultra Panel v1.0 is designed for analyzing genomic composition status with high resolution. This SNP skeleton-based panel can scan the whole genome with 50 Kb apart by targeting over 52,000 high MAF SNP sites.

HiSNP Panel v1.0 is designed for analyzing genomic composition status with basic resolution. This SNP skeleton-based panel can scan the whole genome with 300 Kb apart by targeting over 9,000 high MAF SNP sites.

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Product details
Kit Content
FAQ
Ordering Information
Resources

Feature

High heterozygosity ratio: Comprehensive designing for Europeans, Africans, Americans and Asians (AF=0.05-0.95)
Stable capture performance: GC balanced; unique flanking sequence (verified by WGS)
Appropriate proportionality: Even distribution in whole genome; LD sites excluded
Superior compatibility: Use alone or spike-in other CDS panels

Application

Product

Application

HiSNP Ultra Panel v1.0

HRD Score analysis

CNV

HiSNP Panel v1.0

CNV

Performance

Capture performance of HiSNP-series Panel

Fig 1. Capture performance of HiSNP-series Panel. DNA libraries were prepared using the NadPrep DNA Library Preparation Kit (for MGI) and the NadPrep DNA Library Preparation Kit (for Illumina®), respectively. A. mapping rate, on-target rate；B-C. coverage uniformity and consistency of HiSNP (HiSNP Panel v1.0) and HiSNP Ultra (HiSNP Ultra Panel v1.0) were exhibited, respectively.

Note：Sample type: human genomic DNA, male (Promega, G1471). MGI platform: MGISEQ-2000 with PE100; Illumina platform: NovaSeq 6000 with PE150.

Capture performance of HiSNP Ultra Panel v1.0

Fig 2. Capture performance of HiSNP Ultra Panel v1.0 with HRD standards. DNA libraries were prepared using the NadPrep DNA Library Preparation Kit (for MGI) and the NadPrep DNA Library Preparation Kit (for Illumina®), respectively. A. mapping rate, on-target rat；B-C. coverage uniformity and consistency of HiSNP (HiSNP Panel v1.0) and HiSNP Ultra (HiSNP Ultra Panel v1.0) were exhibited, respectively.。

Note：Sample type: human genomic DNA, male (Promega, G1471). MGI platform: MGISEQ-2000 with PE100; Illumina platform: NovaSeq 6000 with PE150.

Capture Performance of HiSNP Ultra Panel v1.0 with HRD Standard

Fig3. Capture performance of HiSNP Ultra Panel v1.0 with HRD standards. DNA libraries were prepared using the NadPrep DNA Library Preparation Kit (for MGI) A. mapping rate, on-target rat；B-C. coverage uniformity and consistency of HiSNP (HiSNP Panel v1.0) and HiSNP Ultra (HiSNP Ultra Panel v1.0) were exhibited, respectively.

Note：GW3: GW-HRD-3 standards (Genewell); GW9: GW-HRD-9 standards (Genewell); GW12: GW-HRD-12 standards (Genewell). All standards are paired cell lines, including Normal (N) and Tumor (T). MGI platform: MGISEQ-2000 with PE100; Illumina platform: NovaSeq 6000 with PE150.

Fig4. Capture performance of HiSNP Ultra Panel v1.0 with HRD standards. DNA libraries were prepared using the NadPrep DNA Library Preparation Kit (for Illumina®) A. mapping rate, on-target rat；B-C. coverage uniformity and consistency of HiSNP (HiSNP Panel v1.0) and HiSNP Ultra (HiSNP Ultra Panel v1.0) were exhibited, respectively.

Note：Sample type: HRD standards (Genewell).GW3: GW-HRD-3 standards (Genewell); GW9: GW-HRD-9 standards (Genewell); GW12: GW-HRD-12 standards (Genewell). All standards are paired cell lines, including Normal (N) and Tumor (T). MGI platform: MGISEQ-2000 with PE100; Illumina platform: NovaSeq 6000 with PE150.

HiSNP for HRD Score

Fig 5. HRD score analysis of HiSNP Ultra Panel v1.0 with HRD standards by different platform. A. MGI Platform；B. Illumina Platform

Note： Sample type: HRD standards (Genewell).GW3: GW-HRD-3 standards (Genewell); GW9: GW-HRD-9 standards (Genewell); GW12: GW-HRD-12 standards (Genewell). All standards are paired cell lines, including Normal (N) and Tumor (T). MGI platform: MGISEQ-2000 with PE100; Illumina platform: NovaSeq 6000 with PE150.

For research use only. Not for use in diagnostic procedures.

Catalog#	Color Of Tube Cap	Item	Volume	Package/Storage
Catalog#	Color Of Tube Cap	Item	Volume	Package/Storage
1001831		HiSNP Panel v1.0, 96 rxn	415 μL	–20℃
1001838		HiSNP Ultra Panel v1.0, 960 rxn	10×96 rxn（415 μL）	–20℃
1001839		HiSNP Ultra Panel v1.0, 1920 rxn	20×96 rxn（415 μL）	–20℃

Can RNA samples be directly used for library preparation with this kit?

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

What is the recommended input range? How to handle inputs exceeding this range?

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

What is the insert size of this kit? How to select sequencing read length?

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

What is the recommended sequencing data volume for IGTR immune repertoire analysis?

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Is this kit suitable for minimal residual disease (MRD) monitoring?

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.

How to use HiSNP-series Panel with others？

To spike-in use with other panels, please contact Nanodigmbio for technical support.

What is the appropriate sequencing data for HiSNP Ultra Panel?

The sequencing depth of HiSNP Ultra is 75× per Gb theoretically. If you want to get 500× of sequencing depth, it is recommended to achieve 7.5 Gb / library.

Product	Details	Catalog
HiSNP Panel v1.0, 96 rxn	96 rxn	1001831
HiSNP Ultra Panel v1.0, 960 rxn	960 rxn	1001838
HiSNP Ultra Panel v1.0, 1920 rxn	1920 rxn	1001839