NadPrep IGTR Multiplex PCR Library Prep Kit

Catalog Number:

#1300001
#1300002

NadPrep IGTR Multiplex PCR Library Prep Kit is based on the principle of multiplex amplification, enabling efficient enrichment of the complementary determining regions (CDR3) of seven loci for human adaptive immune antigen receptors, including IGH, IGK, IGL, TRA, TRB, TRG and TRD. Combined with high-throughput sequencing technology, this kit facilitates precise detection of B-cell and T-cell diversity and clonality. It is compatible with human DNA or cDNA initial samples from various sources and is widely applicable for immune repertoire studies, clonality analysis of lymphoid malignancies, and minimal residual disease (MRD) monitoring.

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Product details
Kit Content
FAQ
Ordering Information
Resources

Feature

● Modular Primer Design

Provides two independent primer sets: IG Primer Mix (covering IGH/IGL/IGK gene-specific primers) and TR Primer Mix (covering TRA/TRB/TRG/TRD gene-specific primers).
Supports single-tube independent detection (IG or TR only) or dual-tube combined detection (IG + TR).
● Broad Compatibility
Sample types: Compatible with human gDNA or cDNA (first-strand cDNA generated from RNA via reverse transcription).
Input flexibility: Compatible with input amounts ranging from 50 to 2,000 ng.
Sequencing platforms: Direct sequencing on Illumina® platforms or sequencing on MGI platforms after circularization.

Workflow

Performance

Flexible Combination of IG/TR Primer Mixes in Separate Tubes for Precise Amplification of Target Regions

Spiking 0.001% of a mixture of RAMOS and JURKAT cell line gDNA into 2 μg leukocyte-derived gDNA, libraries were prepared using NadPrep IGTR Multiplex PCR Library Prep Kit, with independent amplification (IG-only or TR-only) or combined amplification (IG + TR).

Table 1. Clone list generated by analysis tool.

Figure 1. CDR3 diversity analysis using the NadPrep IGTR Multiplex PCR Library Prep Kit. A. IG Primer Mix (single tube)(on-target: 86.49%); B. TR Primer Mix (single tube) (on-target: 93.36%); C. IG Primer Mix + TR Primer Mix (dual tubes) (on-target: 91.62%).
Note: Sequencing was performed on NovaSeq 6000 (PE150) with data of 2~2.4 Gb for analysis.

#Package	Cap Color	Component	Volume 1300001 (6 rxn)	Volume 1300002 (96 rxn)
Box 1		2X Multiplex PCR Mix	180 μL	2×1,600 μL
		IG Primer Mix	15 μL	230 μL
		TR Primer Mix	15 μL	230 μL
		Multiplex Index Primer Mix #	6×5 μL	24×10 μL
Box 2	/	NadPrep SP Beads	400 μL	6,400 μL

Can RNA samples be directly used for library preparation with this kit?

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

What is the recommended input range? How to handle inputs exceeding this range?

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

What is the insert size of this kit? How to select sequencing read length?

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

What is the recommended sequencing data volume for IGTR immune repertoire analysis?

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Is this kit suitable for minimal residual disease (MRD) monitoring?

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.

Type	Product	Detail	Catalog#
Multiplex PCR Lib Prep	NadPrep IGTR Multiplex Library Prep Kit, 6 rxn	Index #1-6	1300001
Multiplex PCR Lib Prep	NadPrep IGTR Multiplex Library Prep Kit, 96 rxn	Index #1-24	1300002