NadPrep Universal Circularization Kit v2

Catalog Number:

#1002241

The NadPrep Universal Circularization Kit v2 is designed for preparation of a single-stranded circular DNA (ssCirDNA) library for the MGI platform based on DNBSEQ^TMtechnology. The circularization module inside is compatible with the circularization of libraries with single index (SI) adapters and dual index (DI) adapters on MGI platform, and NadPrep Universal Stubby Adapters (UDI), and is suitable for the circularization of libraries converted by the MGIEasy Universal Library Conversion Kit (App-A), with the preparation of single-stranded circular libraries effectively streamlined.

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Feature

● Wide applicability: applicable to many types of library
● High flexibility: compatible with libraries of various types, insert fragment size and input amounts
● Efficient circularization: high circularization efficiency and stability
● Optimized workflow: 1/3 shorter experiment time compared to v1

Workflow

Performance

Simplefied Pipeline without Compromise

Fig 1. Circularization efficiency and NGS QC performance of different types of libraries.Libraries were prepared by using NadPrep DNA Universal Library Preparation Kit (for MGI) coupled with NadPrep Universal Adapter (SI) Module (for MGI), NadPrep Universal Adapter (MDI) Module (for MGI) and NadPrep Universal Stubby Adapter (UDI) Module, and were circularized by using NadPrep v2, Vendor M*_SI, Vendor M*_DI and Vendor M*_Illumina circularization kit, respectively. The A. Circularization efficiency of NadPrep v2 is obviously better than other vendors. There is no significant difference in B. Q30 and C. Mapping rate among these kits on MGI platform (MGISEQ-2000, PE100).
Note：Sample type: Human Female Genomic DNA standard (Promega-Female, G1521); Library input: 1 pmol; NadPrep v2 refers to the libraries circularized by using the NadPrep Universal Circularization Kit v2.

Prepared for Kinds of Libraries

Fig 2. Circularization performance of libraries with various sample types, insert size and input amount. Libraries were prepared by using NadPrep DNA Universal Library Preparation Kit (for MGI) coupled with NadPrep Universal Adapter (SI) Module (for MGI), NadPrep Universal Adapter (MDI) Module (for MGI) and NadPrep Universal Stubby Adapter (UDI) Module, and were circularized by using NadPrep v2, NadPrep v1 and Vendor M* circularization kit, respectively. The Circularization efficiency of A. different types of libraries circularized with NadPrep v2 is obviously better than other circularization kits. There is no significant difference in the circularization efficiency among these libraries with different B. insert size and C. input amount.
Note：NadPrep^® v1: NadPrep^® NadPrep v1 refers to the libraries circularized by using the NadPrep Universal Circularization Kit (for MGI); The input amount in A & B is 1 pmol; The sample type in B & C are PCR products and Human Female Genomic DNA standard (Promega-Female, G1521), respectively; The average insert size in C is 280 bp.

Unbiased GC Coverage

Fig 3. Circularization performance from metagenomic samples. Libraries were prepared by using NadPrep DNA Universal Library Preparation Kit (for MGI) coupled with NadPrep Universal Adapter (SI) Module (for MGI), NadPrep Universal Adapter (MDI) Module (for MGI) and NadPrep Universal Stubby Adapter (UDI) Module, and were circularized by using NadPrep v2 and Vendor M*circularization kit, respectively. The coverage of regions with various GC content, including A. Pseudomonas aeruginosa, B. Salmonella enterica and C. Staphylococcus aureus, are uniform
Note：Sample type: microbial community DNA standard (Zymo Research, D6306).

For research use only. Not for use in diagnostic procedures.

NadPrep Universal Circularization Kit v2

Package	Color Of Tube Cap	Component	Volume 1002241(16 rxn)
Box 1		Universal Splint Buffer II	390 µL
		DNA Ligase	12 µL
		Digestion Enzyme	40 µL
		Stop Solution	140 µL
		TE Solution	1.7 mL
Box 2	/	NadPrep SP Beads	4 mL