NEXome Plus Panel v1.0

Catalog Number:

#1001862
#1001861

NEXome Plus Panel v1.0 is an enhanced whole exome capture Panel. It extends the target regions to SNP skeleton of whole genome, intronic regions related to common gene fusion in solid tumors and classical microsatellite loci on the basis of NEXome Core Panel, which spans a 43.3 Mb region of human genome.

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FAQ
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SNP skeleton of whole genome

Based on the 1000 Genomes database, we mainly select >9,000 loci with high MAF values and high heterozygosity in the Chinese population, covering the entire human genome at approximately 300 Kb intervals. It provides richer and more evenly distributed information on the genomic composition based on the whole exome, and can be used for detection of CNVs and loss of heterozygosity.

Common gene fusion in solid tumors

Contains intron regions and non-coding regions related to common fusions and can be used to analyze fusion loci in solid tumors.

ALK

intron 18 - 19

BCL2

3'UTR

BCR

intron 8, 13 - 14

BRAF

intron 7 - 10

BRCA1

intron 2, 7 - 8, 12, 16, 19 - 20

BRCA2

intron 2

CD74

intron 6 - 8

EGFR

intron 7, 15, 24 - 27

ETV4

intron 5 - 6

ETV5

intron 6 - 7

ETV6

intron 5 - 6

EWSR1

intron 6 - 13

EZR

intron 9 - 12

FGFR1

intron 1, 5, 17

FGFR2

intron 1, 17

FGFR3

intron 17

FLI1

intron 3 - 8

KIT

intron 16

KMT2A

intron 6 - 11

MET

intron 1, 14

MSH2

intron 5

MYB

intron 14

MYC

intron 1

NOTCH2

intron 26

NTRK1

intron 8 - 10

NTRK2

intron 12, 15

NTRK3

intron 13 - 14

NUTM1

intron 1

PDGFB

intron 1

PDGFRA

intron 7, 9, 11

RAF1

intron 4 - 9

RARA

intron 2

RET

intron 7 - 11

ROS1

intron 31 - 35

RSPO2

Upstream, 5'UTR, exon 1 - 2, intron 1

SDC4

intron 2

SLC34A2

intron 4

TMPRSS2

intron 1 - 3

Classical microsatellite loci

The NEXome Core Panel itself covers a large number of microsatellite loci, and the addition of 15 optimally designed MSI classical loci facilitates the comparison between different microsatellite instability analytical methods as well as the optimization and calibration of the analytical process.

BAT-25

BAT-26

BAT-40

BAT-RII

NR-21

NR-22

NR-24

NR-27

MONO-27

D2S123

D5S346

D17S261

D17S520

D17S250

D18S34

Performance

Capture Performance

Figure 1. Capture performance of NEXome Plus Panel v1.0 at 1-plex and 12-plex levels.Libraries were prepared using the NadPrep DNA Universal Library Preparation Kit (for Illumina®). NEXome Plus Panel v1.0 were used to complete hybridization capture. Sequencing platform: Illumina HiSeq X Ten, PE150. The BWA was used for alignment to the reference genome hg38, and on-target rate was calculated according to reads number.
Note：The samples: Human Male Genomic DNA (Promega-Male, G1471) and Human Female Genomic DNA (Promega-Female, G1521).

Figure 2. Capture performance of NEXome Plus Panel v1.0 in different gDNA libraries.Libraries were prepared using the NadPrep DNA Universal Library Construction Kit (for Illumina®). NEXome Plus Panel v1.0 (12 plex) were used to complete hybridization capture. Ssequencing platform: Illumina HiSeq X Ten with PE150. The BWA was used for alignment to the reference genome hg38, and on-target rate was calculated according to reads number. A. Mappability and on-target rate; B. Target covered; C. Coverage uniformity and consistency; D. GC bias.

For research use only. Not for use in diagnostic procedures.

Catalog#	Color of Tube Cap	Product	Volume	Package/Storage
1001861		NEXome Plus Panel v1.0, 96 rxn	415 μL	–20℃
1001862		NEXome Plus Panel v1.0, 16 rxn	70 μL	–20℃

Can RNA samples be directly used for library preparation with this kit?

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

What is the recommended input range? How to handle inputs exceeding this range?

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

What is the insert size of this kit? How to select sequencing read length?

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

What is the recommended sequencing data volume for IGTR immune repertoire analysis?

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Is this kit suitable for minimal residual disease (MRD) monitoring?

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.