No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.

Figure 2. Correlation of ERCC expression profiles in captured libraries from RNA samples processed through different library preparation protocols. A. NadPrep RNA & DNA Library Co-Preparation Module; B. NadPrep Total RNA-To-DNA Module conjunction NadPrep DNA Library Preparation Module.

Note: The samples originated from K562 cell line RNA with an initial input of 50 ng; the pre-library input amount was 200 ng.

Figure 1. Library yield and capture performance of DNA samples using different library preparation protocols. A. Library Yield; B. Mappability.

Note: The sample originated from 50 ng of Human Genomic DNA Standard (Promega, G1521). Sequencing mode was Illumina NovaSeq 6000 platform, PE150.

Libraries prepared with this module can be used in conjunction with NEX-t Panel v1.0 and NadPrep ES Hybrid Capture Reagents. This significantly shortens the time required for pathogen-targeted sequencing and simplifies experimental procedures.

When co-prepare RNA & DNA libraries from mixed nucleic acid samples, a higher proportion of DNA samples results in larger fragments with enzymatic digestion. Adjustments can be made according to the expected insert fragment lengths and recommended time of enzymatic fragmentation in the manual.

This module can tolerate nucleic acid samples containing 6 μL of EDTA (1 mM), resulting in a final concentration of 0.3 mM in the cDNA first-strand synthesis system. At this concentration, RNA reverse transcription inhibition is minimal, and the efficiency of enzymatic digestion is unaffected. Before library preparation, confirm the sample solvent. If it exceeds the recommended EDTA threshold, it is necessary to purify nucleic acid samples using 2X NadPrep SP Beads, replacing the elution reagent with Nuclease Free Water before proceeding with library preparation.