HGBP Panel v1.0

Catalog Number:

#1001961 (96 rxn)
#1001962 (16 rxn)

HGBP Panel v1.0 targets human hemoglobin coding genes, adjacent genomic regions, and regulatory genes. The panel covers a 160 Kb genomic region for enrichment of information on multiple variants associated with human hemoglobin.

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Performance

Capture Performance

Figure 1. Capture performance of HGBP Panel v1.0. The Libraries were prepared with 50 ng standard gDNA, using the NadPrep DNA Universal Library Preparation Kit (for Illumina®). The hybridization reaction (6-plex) was initiated with 500 ng input of each library. The capture was performed by using HGBP Panel v1.0, followed by sequencing using Novaseq 6000 PE150.

Note：Samples gDNA_1-6 were thalassemia gDNA standards (GeneWell), i.e., GW-TGTS001, GW-TGTS006, GW-TGTS009, GW-TGTS027, GW-TGTS028 and GW- TGTS023, respectively.

Multiple Variants Analysis

Table 1. Variant analysis results of thalassemia gDNA standards through targeted capture using HGBP Panel v1.0.

Following targeted capture of standards using HGBP Panel v1.0, sequencing was performed using Illumina® platform, and base substitutions and InDel, known bre- akpoints, and copy number variations were analyzed using vardict, delly, and CNVkit, respectively.

Note：Note: Blue indicated the frequency of variation; green indicated the ratio of split reads, * indicated that split reads cannot be identified. split reads

Figure 2. Copy number of α gene cluster segments in thalassemia gDNA standards through targeted capture using HGBP Panel v1.0.As shown in figure above, copy number analysis still visually reflected the copy number of each gene in the region when the breakpoint location failed to be captured or the recombi- nant sequence cannot be distinguished.

Note：Samples are thalassemia gDNA standards (GeneWell, GW-TGTS006).

For research use only. Not for use in diagnostic procedures.

Catalog

Color of Cap Tube

Product

Volume

Packing/Storage Temperature

1001961

HGBP Panel v1.0, 96 rxn

415 μL

–20℃

1001962

HGBP Panel v1.0, 16 rxn

70 μL

–20℃

Can RNA samples be directly used for library preparation with this kit?

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

What is the recommended input range? How to handle inputs exceeding this range?

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

What is the insert size of this kit? How to select sequencing read length?

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

What is the recommended sequencing data volume for IGTR immune repertoire analysis?

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Is this kit suitable for minimal residual disease (MRD) monitoring?

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.