HGBP Panel v1.0 targets human hemoglobin coding genes, adjacent genomic regions, and regulatory genes. The panel covers a 160 Kb genomic region for enrichment of information on multiple variants associated with human hemoglobin.
Capture Performance
Figure 1. Capture performance of HGBP Panel v1.0. The Libraries were prepared with 50 ng standard gDNA, using the NadPrep DNA Universal Library Preparation Kit (for Illumina®). The hybridization reaction (6-plex) was initiated with 500 ng input of each library. The capture was performed by using HGBP Panel v1.0, followed by sequencing using Novaseq 6000 PE150.
Note:Samples gDNA_1-6 were thalassemia gDNA standards (GeneWell), i.e., GW-TGTS001, GW-TGTS006, GW-TGTS009, GW-TGTS027, GW-TGTS028 and GW- TGTS023, respectively.
Following targeted capture of standards using HGBP Panel v1.0, sequencing was performed using Illumina® platform, and base substitutions and InDel, known bre- akpoints, and copy number variations were analyzed using vardict, delly, and CNVkit, respectively.
Note:Note: Blue indicated the frequency of variation; green indicated the ratio of split reads, * indicated that split reads cannot be identified. split reads
Figure 2. Copy number of α gene cluster segments in thalassemia gDNA standards through targeted capture using HGBP Panel v1.0.As shown in figure above, copy number analysis still visually reflected the copy number of each gene in the region when the breakpoint location failed to be captured or the recombi- nant sequence cannot be distinguished.
Note:Samples are thalassemia gDNA standards (GeneWell, GW-TGTS006).
Catalog |
Color of Cap Tube
|
Product |
Volume
|
Packing/Storage Temperature |
1001961 |
|
HGBP Panel v1.0, 96 rxn |
415 μL |
–20℃ |
1001962 |
|
HGBP Panel v1.0, 16 rxn |
70 μL |
–20℃ |
No. This kit is
compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse
transcription to generate cDNA is required prior to library preparation.
This kit supports
50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the
sample into multiple reactions to maintain amplification efficiency.
The main peak of
PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for
high-quality coverage.
A minimum of 0.3
Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng.
Increase data volume to enhance sensitivity for low-frequency clones.
Yes. This kit
includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in
separate tubes, allowing flexible combination in a single amplification
reaction. With its high sensitivity, the kit meets the requirements for MRD
monitoring technology development and clinical applications, making it ideal
for low-frequency variant detection scenarios.
Product | Catalog |
HGBP Panel v1.0, 96rxn | 1001961 |
HGBP Panel v1.0, 16rxn | 1001962 |
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