The NadPrep NanoBlockers (for Illumina®) are universal blockers for Illumina platforms. NadPrep NanoBlockers act by reducing non-specific binding of adapter sequences, which improves on-target rate and increases enrichment depth. NadPrep NanoBlockers (for Illumina®) work with Illumina libraries with single/dual indexes and 6nt/8nt indexes.
Feartures
Performance
Figure 1. Performance of NadPrep NanoBlockers(for Illumina®). The NadPrep NanoBlockers (for Illumina®) worked effectively in libraries with different input amount. Libraries were prepared using NadPrep DNA Library Preparation Module (for Illumina®) with 500 ng and 6000 ng input separately.
Note: Sample type: gDNA, sequencing platform: MGISEQ-2000, PE100; BWA was used to align raw reads to reference genome (GRCh37, hg19) and calculate on-target rates by using reads aligned to the panel regions and flanking sequences.
For research use only. Not for use in diagnostic procedures.
Catalog# |
Color Of Tube Cap |
Item |
Volume |
Package/Storage |
1006101 |
|
NadPrep NanoBlockers (for Illumina), 16 rxn |
40 μl×1 |
–20℃ |
1006102 |
|
NadPrep NanoBlockers (for Illumina), 96 rxn |
210 μl×1 |
–20℃ |
No. This kit is
compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse
transcription to generate cDNA is required prior to library preparation.
This kit supports
50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the
sample into multiple reactions to maintain amplification efficiency.
The main peak of
PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for
high-quality coverage.
A minimum of 0.3
Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng.
Increase data volume to enhance sensitivity for low-frequency clones.
Yes. This kit
includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in
separate tubes, allowing flexible combination in a single amplification
reaction. With its high sensitivity, the kit meets the requirements for MRD
monitoring technology development and clinical applications, making it ideal
for low-frequency variant detection scenarios.
Product |
Catalog# |
NadPrep NanoBlockers (for Illumina®), 16 rxn |
1006101 |
NadPrep NanoBlockers (for Illumina®), 96 rxn |
1006102 |
For research use only. Not for use in diagnostic procedures.
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If there are any problems, please contact us by 400 8717 699 / support@njnad.com