NadPrep NanoBlockers (for Illumina®)

Catalog Number:

#1006101 #1006102

The NadPrep NanoBlockers (for Illumina®) are universal blockers for Illumina platforms. NadPrep NanoBlockers act by reducing non-specific binding of adapter sequences, which improves on-target rate and increases enrichment depth. NadPrep NanoBlockers (for Illumina®) work with Illumina libraries with single/dual indexes and 6nt/8nt indexes.

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Product details
Kit Content
FAQ
Ordering Information
Resources

Feartures

Universal for different Truseq®and compatible with varieties of Illumina® platforms;
Effectively reduce non-specific binding of adapter sequences and significantly increase the on-target rate and increase enrichment depth;
Pre-mixed solution

Performance

Figure 1. Performance of NadPrep NanoBlockers(for Illumina®). The NadPrep NanoBlockers (for Illumina®) worked effectively in libraries with different input amount. Libraries were prepared using NadPrep DNA Library Preparation Module (for Illumina®) with 500 ng and 6000 ng input separately.
Note: Sample type: gDNA, sequencing platform: MGISEQ-2000, PE100; BWA was used to align raw reads to reference genome (GRCh37, hg19) and calculate on-target rates by using reads aligned to the panel regions and flanking sequences.

For research use only. Not for use in diagnostic procedures.

Catalog#	Color Of Tube Cap	Item	Volume	Package/Storage
1006101		NadPrep NanoBlockers (for Illumina), 16 rxn	40 μl×1	–20℃
1006102		NadPrep NanoBlockers (for Illumina), 96 rxn	210 μl×1	–20℃

Can RNA samples be directly used for library preparation with this kit?

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

What is the recommended input range? How to handle inputs exceeding this range?

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

What is the insert size of this kit? How to select sequencing read length?

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

What is the recommended sequencing data volume for IGTR immune repertoire analysis?

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Is this kit suitable for minimal residual disease (MRD) monitoring?

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.