LungCancer Panel

Catalog Number:

#1001922 (16 rxn)
#1001921 (96 rxn)

LungCancer Panel v1.0 is specifically designed for lung cancer research. This panel involves 24 genes, including full coding regions from 19 genes and non-exonic regions with frequent fusion occurrence in lung cancer.

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FAQ
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Gene List

Genes with coding regions covered

*ALK*	*BRAF*	*CD274*	CD74	*EGFR*	*ERBB2*	EZR	*FGFR1*	*FGFR2*	*FGFR3*	*KRAS*	*MET*
*NRAS*	*NTRK1*	*NTRK2*	*NTRK3*	NUTM1	*PDCD1LG2*	*PDGFRA*	*PIK3CA*	*RET*	*ROS1*	SDC4	SLC34A2

Genes with full coding regions shown in bold.

Performance

Table 1. Performance of LungCancer Panel v1.0

Platform

On-target rate (%)

Duplication (%)

% target

> 0.2 x mean

% target

> 0.5 x mean

% target

> 1 x mean

Fold 80 base penalty

MGI

92.75

6.20

99.21

96.21

52.12

1.28

Illumina^®️

93.98

12.21

99.98

97.92

52.14

1.27

The DNA libraries were prepared from human genomic DNA (Promega, G1521). 1 M read pairs were subsampled for the analysis.

Fig 1. Evaluation of LungCancer Panel v1.0 on the analysis of SNVs and indels. The DNA libraries were prepared from Onco SNV Multiplex 1-25% gDNA (GeneWell, GW-OGTM004) using NadPrep library kits. The enriched libraries were sequenced either on HiSeq X Ten (PE150) or DNBSEQ-G400 (PE100).

For research use only. Not for use in diagnostic procedures.

Catalog#	Color Of Tube Cap	Item	Volume	Package/Storage
1001922		LungCancer Panel v1.0, 16 rxn	70 μl×1	–20℃
1001921		LungCancer Panel v1.0, 96 rxn	415 μl×1	–20℃

Can RNA samples be directly used for library preparation with this kit?

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

What is the recommended input range? How to handle inputs exceeding this range?

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

What is the insert size of this kit? How to select sequencing read length?

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

What is the recommended sequencing data volume for IGTR immune repertoire analysis?

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Is this kit suitable for minimal residual disease (MRD) monitoring?

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.