NadPrep DNA Library Preparation Kit v2

Catalog Number:

#1002421
#1002422

NadPrep DNA Library Preparation Module v2 is designed for the preparation of high-quality libraries from double-stranded DNA (dsDNA) on Illumina® & MGI platforms. This A-T ligation-based kit offers a stable and efficient library preparation solution for ap plications including whole genome sequencing of 1‒500 ng DNA and hybridization capture based targeted sequencing. Comp ared to the v1 version, the upgraded kit has improved the library conversion efficiency and better tolerance for low-quality sam ples. Consequently, it can enhance library complexity while maintaining high fidelity.

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Product Feature

● Fast and Convenient
Complete the entire process of whole-genome library preparation within 3 hr
● Stable and Efficient

Higher library yield
Higher library conversion rate

Low GC bias

Higher Library Complexity

● Flexible Compatibility
Flexible selection of multiple types of adapters to accommodate both MGI and Illumina platforms
Compatible with input amounts ranging from 1 ng to 500 ng
Compatible with various sample types such as gDNA, FFPE DNA, cfDNA, etc

Compatible with liquid-phase hybridization capture

Workflow

Performance

Stable and Efficient Library Yield

Compatible with a wide range of input amounts

Figure 1. Library yield of NadPrep DNA Library Preparation Kit v2 for gDNA samples with different input amounts. The libraries were prepared by using NadPrep DNA Library Preparation Module v2 coupled with NadPrep Universal Adapter (MDI) Module (for MGI) and NadPrep Universal Stubby Adapter (UDI) Module. A. Library yield (MGI); B. Library yield (Illumina).

Note: gDNA samples are human genomic DNA standard (Promega, G1521).

Compatible with various types of samples

Figure 2. Library yield of NadPrep DNA Library Preparation Kit v2 for different types of samples. The libraries were prepared by using NadPrep DNA Li brary Preparation Module v2 coupled with A. NadPrep Universal Stubby Adapter (UDI) Module and B. NadPrep BMI Adapter (MDI) Module (for MGI), and NadPrep UMI Adapter Kit (with 10 nt Index), respectively. The number of amplification cycles followed the corresponding user manuals.
Note: The grading standards by electrophoresis are as follows: gDNA: one bright and non-trailing stripe with about 15 kb in size; FFPE C: multiple stripes ranging from 200 bp to 2,500 bp, with severe diffusion.

Compatible with different types of adapters

Figure 3. Library yield of NadPrep DNA Library Preparation Kit v2 with di fferent types of adapters. The libraries were prepared from different input sa mples by using NadPrep DNA Library Preparation Kit/Module (for Illumina®/MGI) (NadPrep v1) and NadPrep DNA Library Preparation Module v2 (NadPrep v2), coupled with NadPrep Universal Stubby Adapter (UDI) Module [Universal Stubby (UDI)], NadPrep Universal Adapter (MDI) Module (for MGI) [MDI], NadPrep BMI Adapter (MDI) Module (for MGI) [BMI], and NadPrep UMI Adapter Kit (with 10 nt Index) [UMI].

Note: gDNA samples are human genomic DNA standard (Promega, G1471).

Low GC Bias

Figure 4. Low GC bias in metagenomic samples library preparation using NadPrep DNA Library Preparation Kit v2. GC bias of whole-genome libraries with various GC content such as A. Staphylococcus aureus, B. Escherichia coli, and C. Pseudomonas aeruginosa.

Higher Library Conversion Rate

Figure 5. Library conversion rate of NadPrep DNA Library Preparation Kit v2. 10 ng plasma cfDNA were prepared by using NadPrep DNA Library Prepara tion Module v2 coupled with NadPrep BMI Adapter (MDI) Module (for MGI) and NadPrep UMI Adapter Kit (with 10 nt Index). The capture was performed by using a 2 Kb Panel with μCaler Hybrid System. Sequencing platform: DNBSEQ-T7, PE150; Illumina Novaseq 6000, PE150. For each sample, 1.0 Gb of data was randomly selected for data analysis, and filtering was performed based on Duplex Conse nsus Sequences (DCS211).

Note: The relative library conversion rate refers to the ratio of library conversion rates achieved by NadPrep v2 and competitor library preparation kits to that of NadPrep DNA Library Preparation Kit/Module (for Illumina®/MGI) (NadPrep v1), with the library conversion rate of NadPrep v1 normalized to 1).

Higher Library Complexity

Figure 6. Library complexity of NadPrep DNA Library Preparation Kit v2. 10 ng plasma cfDNA were prepared by using NadPrep DNA Library Preparation Module v2 coupled with NadPrep UMI Adapter Kit (with 10 nt Index). The capture was performed using a 2 Kb Panel with μCaler hybrid system. Sequencing plat form: Illumina Novaseq 6000, PE150. For each sample, 1.0 Gb of data was rand omly selected for data analysis, and filtering was performed based on Duplex Consensus Sequences (DCS211).

Compatible with Liquid-phase Hybridization Capture

Figure 7. The capture performance of pre-libraries prepared by NadPrep DNA Library Preparation Kit v2. The libraries were prepared from 50 ng FFPE C grade DNA samples by using NadPrep DNA Library Preparation Module v2, and the multi-hybridization reaction was initiated with 500 ng of each pre-library by using LungCancer Panel v1.0 (0.23 Mb) and NanOnco Plus Panel v3.0 (2.4 Mb) coupled with NadPrep Hybrid Capture Reagents. A. Mappability and On-target rate; B. Target covered; C. Fold 80 base penalty; D. Average sequencing depth after deduplication.

Analysis of Mutation in Standard

Figure 8. Consistency of the allele frequencies with the nominal frequencies of the standards in captured data from the libraries prepared by NadPrep DNA Library Preparation Kit v2. The libraries were prepared by using NadPrep DNA Library Preparation Module v2 coupled with NadPrep Universal Stubby Adapter (UDI) Module, with 6 cycles of amplification. The capture was performed using NadPrep Hybrid Capture Reagents with the LungCancer Panel v1.0 (0.23 Mb), with a hybridization time of 16 hr. Sequencing platform: Illumina Novaseq 6000, PE 150.

Note: The samples used were Onco Structural Multplex 5% aDNA (Genewell. GW-OGTM001), with an initial input of 50 ng.

Library Prep Module

Package	Color of Cap Tube	Component	Volume 1002421(24 rxn)	Volume 1002422 (96 rxn)
Box1		EndRepair & A-Tailing Buffer Pro	180 µL	690 µL
		End Repair & A-Tailing Enzyme Pro	120 µL	460µL
		Ligation Buffer	750 µL	2×1500 µL
		DNA Ligase	65 µL	250 µL
		2X HiFi PCR Master Pro Mix	720 µL	2×1600 µL
	/	Nuclease Free Water	2.5 mL	8 mL
		TE Solution	1 mL	4 mL
Box2	/	NadPrep SP Beads	4 mL	15 mL

Universal Adapter (SI) Module (for MGI)

Color of Cap Tube	Component	Volume 1003611 (24 rxn)	Volume 1003621 (96 rxn)	Volume 1003622(96 rxn)
	NadPrep M-Index (SI) Primer Mix #	12×15 µL (Tube)	24×25 µL (Tube)	24×25 µL (Tube)
	NadPrep M-Adapter (SI)	60 µL (Tube)	230 µL (Tube)	230 µL (Tube)

Color of Cap Tube	Component	Volume 1003641 (96 rxn)
Color of Cap Tube	Component	Volume 1003641 (96 rxn)
	NadPrep M-Index (SI) Primer Mix #	96×8 µL (Plate)
	NadPrep M-Adapter (SI)	260 µL (Tube)
	NadPrep M-Amplification Primer Mix (for MGI, SI)	75 µL (Tube)

Universal Adapter (MDI) Module (for MGI)

Color of Cap Tube	Component	Volume 1003711 (24 rxn)	Volume 1003721 (96 rxn)	Volume 1003722 (96 rxn)	Volume 1003725 (96 rxn)	Volume 1003726 (96 rxn)
	NadPrep M-Index (MDI) Primer Mix #	12×15 µL (Tube)	24×25 µL (Tube)	24×25 µL (Tube)	24×25 µL (Tube)	24×25 µL (Tube)
	NadPrep M-Adapter (DI)	60 µL (Tube)	230 µL (Tube)	230 µL (Tube)	230 µL (Tube)	230 µL (Tube)

Color of Cap Tube	Component	Volume 1003751 (96 rxn)	Volume 1003752 (96 rxn)	Volume 1003753 (96 rxn)	Volume 1003754 (96 rxn)
Color of Cap Tube	Component	Volume 1003751 (96 rxn)	Volume 1003752 (96 rxn)	Volume 1003753 (96 rxn)	Volume 1003754 (96 rxn)
	NadPrep M-Index (MDI) Primer Mix #	96×8 µL (Plate)	96×8 µL (Plate)	96×8 µL (Plate)	96×8 µL (Plate)
	NadPrep M-Adapter (DI)	260 µL (Tube)	260 µL (Tube)	260 µL (Tube)	260 µL (Tube)
	NadPrep M-Amplification Primer Mix (for MGI, DI)	75 µL (Tube)	75 µL (Tube)	75 µL (Tube)	75 µL (Tube)

Color of Cap Tube	Component	Volume 1003761 (1152 rxn)	Volume 1003762 (1152 rxn)	Volume 1003763 (1152 rxn)	Volume 1003764 (1152 rxn)
Color of Cap Tube	Component	Volume 1003761 (1152 rxn)	Volume 1003762 (1152 rxn)	Volume 1003763 (1152 rxn)	Volume 1003764 (1152 rxn)
	NadPrep M-Index (MDI) Primer Mix #	96×72 µL (Tube)	96×72 µL (Tube)	96×72 µL (Tube)	96×72 µL (Tube)
	NadPrep M-Adapter (DI)	2×1350 µL (Tube)	2×1350 µL (Tube)	2×1350 µL (Tube)	2×1350 µL (Tube)
	NadPrep M-Amplification Primer Mix (for MGI, DI)	900 µL (Tube)	900 µL (Tube)	900 µL (Tube)	900 µL (Tube)

Universal Stubby Adapter (UDI) Module (for Illumina)

Color of Cap Tube	Component	Volume 1003240 (24 rxn)	Volume 1003241 (96 rxn)	Volume 1003242 (96 rxn)
	NadPrep Universal UDI-index Primer Mix #	12×15 µL (Tube)	24×25 µL (Tube)	24×25 µL (Tube)
	NadPrep Universal Stubby Adapter	60 µL (Tube)	230 µL (Tube)	230 µL (Tube)
	NadPrep Amplification Primer Mix II	20 µL (Tube)	75 µL (Tube)	75 µL (Tube)

Color of Cap Tube	Component	Volume 1003251 (96 rxn)	Volume 1003252 (96 rxn)	Volume 1003253 (96 rxn)	Volume 1003254 (96 rxn)
Color of Cap Tube	Component	Volume 1003251 (96 rxn)	Volume 1003252 (96 rxn)	Volume 1003253 (96 rxn)	Volume 1003254 (96 rxn)
	NadPrep Universal UDI-index Primer Mix #	96×8 µL (Plate)	96×8 µL (Plate)	96×8 µL (Plate)	96×8 µL (Plate)
	NadPrep Universal Stubby Adapter	260 µL (Tube)	260 µL (Tube)	260 µL (Tube)	260 µL (Tube)
	NadPrep Amplification Primer Mix II	75 µL (Tube)	75 µL (Tube)	75 µL (Tube)	75 µL (Tube)

Color of Cap Tube	Component	Volume 1003261 (1152 rxn)	Volume 1003262 (1152 rxn)	Volume 1003263 (1152 rxn)	Volume 1003264 (1152 rxn)
Color of Cap Tube	Component	Volume 1003261 (1152 rxn)	Volume 1003262 (1152 rxn)	Volume 1003263 (1152 rxn)	Volume 1003264 (1152 rxn)
	NadPrep Universal UDI-index Primer Mix #	96×72 µL (Tube)	96×72 µL (Tube)	96×72 µL (Tube)	96×72 µL (Tube)
	NadPrep Universal Stubby Adapter	2×1350 µL (Tube)	2×1350 µL (Tube)	2×1350 µL (Tube)	2×1350 µL (Tube)
	NadPrep Amplification Primer Mix II	900 µL (Tube)	900 µL (Tube)	900 µL (Tube)	900 µL (Tube)

UDI Adapter Module (for Illumina®)

Color of Cap Tube	Component	Volume 1003211 (24 rxn)	Volume 1003212 (24 rxn)	Volume 1003211 (96 rxn)	Volume 1003212 (96 rxn)	Volume 1003213 (192 rxn)	Volume *1003224 (480 rxn)**
	NadPrep UDI Adapter #	12×10 µL (Tube)	12×10 µL (Tube)	12×22 µL (Tube)	12×22 µL (Tube)	24×22 µL (Tube)	96×3.5 µL (Plate)×5

*The packaging form is 96 rxn/Plate。

BMI Adapter (SI) Module (for MGI)

Color of Cap Tube	Component	Volume 1003811 (24 rxn)	Volume 1003821 (96 rxn)	Volume 1003822(96 rxn)
	NadPrep M-Index (SI) Primer Mix #	12×15 µL	24×25 µL	24×25 µL
	NadPrep BMI-Adapter	60 µL	230 µL	230 µL

Package	Color of Cap Tube	Component	Volume 1003820 (480 rxn)
Box1		NadPrep M-Index (SI) Primer Mix #	96×30 µL
Box2		NadPrep BMI-Adapter	1100 µL

BMI Adapter (MDI) Module (for MGI)

Color of Cap Tube	Component	Volume 1003911 (24 rxn)	Volume 1003921 (96 rxn)	Volume 1003922(96 rxn)	Volume 1003925 (96 rxn)	Volume 1003926 (96 rxn)
	NadPrep M-Index (MDI) Primer Mix #	12×15 µL	24×25 µL	24×25 µL	24×25 µL	24×25 µL
	NadPrep BMI-Adapter	60 µL	230 µL	230 µL	230 µL	230 µL

UMI Adapter Module（for Illumina）

Color of Cap Tube	Component	Volume 1103111 (24 rxn)	Volume 1103121 (96 rxn)	Volume 1103122 (96 rxn)
	NadPrep Universal UDI-Index Primer Mix #	12×15 µL	24×25 µL	24×25 µL
	NadPrep UMI Adapter	60 µL	230 µL	230 µL
	NadPrep Amplification Primer Mix II	20 µL	75 µL	75 µL

Type	Product	Detail	Catalog#
Library Prep Module	NadPrep DNA Library Preparation Module v2, 24 rxn	24 rxn	1002421
Library Prep Module	NadPrep DNA Library Preparation Module v2, 96 rxn	96 rxn	1002422
Universal Adapter (SI) Module (for MGI)	NadPrep Universal Adapter (SI) Module Set A1 (for MGI), 24 rxn	SI 1-12	1003611
	NadPrep Universal Adapter (SI) Module Set E1 (for MGI), 96 rxn	SI 1-96	1003641
	NadPrep Universal Adapter (SI) Module Set B1 (for MGI), 96 rxn	SI 1-24	1003621
	NadPrep Universal Adapter (SI) Module Set B2 (for MGI), 96 rxn	SI 25-48	1003622
Universal Adapter (MDI) Module (for MGI)	NadPrep Universal Adapter (MDI) Module Set A1 (for MGI), 24 rxn	MDI 1-12	1003711
	NadPrep Universal Adapter (MDI) Module Set B1 (for MGI), 96 rxn	MDI 1-24	1003721
	NadPrep Universal Adapter (MDI) Module Set B2 (for MGI), 96 rxn	MDI 25-48	1003722
	NadPrep Universal Adapter (MDI) Module Set B3 (for MGI), 96 rxn	MDI 49-72	1003725
	NadPrep Universal Adapter (MDI) Module Set B4 (for MGI), 96 rxn	MDI 73-96	1003726
	NadPrep Universal Adapter (MDI) Module Set E1 (for MGI), 96 rxn	MDI 1-96	1003751
	NadPrep Universal Adapter (MDI) Module Set E2 (for MGI), 96 rxn	MDI 97-192	1003752
	NadPrep Universal Adapter (MDI) Module Set E3 (for MGI), 96 rxn	MDI 193-288	1003753
	NadPrep Universal Adapter (MDI) Module Set E4 (for MGI), 96 rxn	MDI 289-384	1003754
	NadPrep Universal Adapter (MDI) Module Set F1 (for MGI), 1152 rxn	MDI 1-96	1003761
	NadPrep Universal Adapter (MDI) Module Set F2 (for MGI), 1152 rxn	MDI 97-192	1003762
	NadPrep Universal Adapter (MDI) Module Set F3 (for MGI), 1152 rxn	MDI 193-288	1003763
	NadPrep Universal Adapter (MDI) Module Set F4 (for MGI), 1152 rxn	MDI 289-384	1003764
Universal Stubby Adapter (UDI) Module	NadPrep Universal Stubby Adapter (UDI) Module Set A1, 24 rxn	Index 1-12 24 rxn	1003240
	NadPrep Universal Stubby Adapter (UDI) Module Set B1, 96 rxn	Index 1-24 96 rxn	1003241
	NadPrep Universal Stubby Adapter (UDI) Module Set B2, 96 rxn	Index 25-48 96 rxn	1003242
	NadPrep Universal Stubby Adapter (UDI) Module Set E1, 96 rxn	Index 1-96 96 rxn	1003251
	NadPrep Universal Stubby Adapter (UDI) Module Set E2, 96 rxn	Index 97-192 96 rxn	1003252
	NadPrep Universal Stubby Adapter (UDI) Module Set E3, 96 rxn	Index 193-288 96 rxn	1003253
	NadPrep Universal Stubby Adapter (UDI) Module Set E4, 96 rxn	Index 289-384 96 rxn	1003254
	NadPrep Universal Stubby Adapter (UDI) Module Set F1, 1152 rxn	Index 1-96 1152 rxn	1003261
	NadPrep Universal Stubby Adapter (UDI) Module Set F2, 1152 rxn	Index 97-192 1152 rxn	1003262
	NadPrep Universal Stubby Adapter (UDI) Module Set F3, 1152 rxn	Index 193-288 1152 rxn	1003263
	NadPrep Universal Stubby Adapter (UDI) Module Set F4, 1152 rxn	Index 289-384 1152 rxn	1003264
UDI Adapter Module (for Illumina®)	NadPrep UDI Adapter Kit, Set A (for Illumina®)	Index 1-12 300 pmol / Adapter	1003211
	NadPrep UDI Adapter Kit, Set B (for Illumina®)	Index 13-24 300 pmol /Adapter	1003212
	NadPrep UDI Adapter Kit, Set A&B (for Illumina®)	Index 1-24 300 pmol / Adapter	1003213
	NadPrep UDI Adapter Kit Set C1 (for Illumina®️)	24 rxn	1003221
	NadPrep UDI Adapter Kit Set D1 (for Illumina®️)	480 rxn	1003224
BMI/UMI Adapter Module	NadPrep BMI Adapter (SI) Module Set A1 (for MGI), 24 rxn	SI 1-12	1003811
	NadPrep BMI Adapter (SI) Module Set B1 (for MGI), 96 rxn	SI 1-24	1003821
	NadPrep BMI Adapter (SI) Module Set B2 (for MGI), 96 rxn	SI 25-48	1003822
	NadPrep BMI Adapter (SI) Module Set D1 (for MGI), 480 rxn	SI 1-96	1003820
	NadPrep BMI Adapter (MDI) Module Set A1 (for MGI), 24 rxn	MDI 1-12	1003911
	NadPrep BMI Adapter (MDI) Module Set B1 (for MGI), 96 rxn	MDI 1-24	1003921
	NadPrep BMI Adapter (MDI) Module Set B2 (for MGI), 96 rxn	MDI 25-48	1003922
	NadPrep BMI Adapter (MDI) Module Set B3 (for MGI), 96 rxn	MDI 49-72	1003925
	NadPrep BMI Adapter (MDI) Module Set B4 (for MGI), 96 rxn	MDI 73-96	1003926
	NadPrep UMI Adapter Kit Set A1 (with 10 nt Index), 24 rxn	1-12	1103111
	NadPrep UMI Adapter Kit Set B1 (with 10 nt Index), 96 rxn	1-24	1103121
	NadPrep UMI Adapter Kit Set B2 (with 10 nt Index), 96 rxn	25-48	1103122