μCaler Total Solution for SARS-CoV-2

Catalog Number:

#1106102 #1106101
#1105102 #1105101
#1101301

μCaler Hybrid Capture Reagents that have been optimized for targeted enrichment of small panels and, coupled with μCaler NanoBlockers and μCaler Panel based on Nanodigmbio’s independent intellectual property rights with innovative scheme design have constituted a complete liquid hybridization capture system, thus enabling the whole process completion of capture-library preparation in same day.

The μCaler SARS-Cov-2 Panel is designed based on the Wuhan-Hu-1/2019 (MN908947) reference genome with dependence on the μCaler probe design scheme. It covers all 29.9 Kb genomic regions and can be used for precise typing, variant tracking, and identification of new variants of SARS-CoV-2.

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Workflow

workflow0209

Features

● Simplified process for easier operation

●Complete the whole process from samples to capture-library preparation in same day

● Small panel has stable and excellent capture performance

Performance

Capture performance and genome-wide coverage of cell-passaged virus

Figure 1. μCaler Capture performance and genome-wide coverage of μCaler SARS-CoV-2 Panel on cell-passaged SARS-CoV-2 variants (Omicron BA.1.1). NadPrep Total RNA-To-DNA ModuleLibraries were prepared with RNA samples using the NadPrep Total RNA-To-DNA Module coupled with the NadPrep DNA Library Preparation Kit (for Illumina®), and a complete hybrid capture solution was developed using the μCaler SARS-CoV-2 Panel coupled with the μCaler Hybrid Capture Reagents and μCaler NanoBlockers (for Illumina®), with sequencing mode of MiniSeq SE 100. The sequencing data were analyzed using IRMA. A. Capture performance of the μCaler SARS-CoV-2 Panel on cell-passaged SARS-CoV-2; B. The genome-wide coverage of SARS-CoV-2.

Capture performance and genome-wide coverage of oropharyngeal swab samples

Figure2. μCaler Capture performance and genome-wide coverage of μCaler SARS-CoV-2 Panel on oropharyngeal swab specimen collected. Libraries were prepared with RNA samples using the NadPrep Total RNA-To-DNA Module coupled with the NadPrep DNA Library Preparation Kit (for Illumina®), and a complete hybrid capture solution was developed using the μCaler SARS-CoV-2 Panel coupled with the μCaler Hybrid Capture Reagents and μCaler NanoBlockers (for Illumina®), with sequencing mode of MiniSeq SE 100. The sequencing data were analyzed using IRMA. A. Capture performance of the μCaler SARS-CoV-2 Panel on oropharyngeal swab specimen collected; B. The genome-wide coverage of SARS-CoV-2.

Genotyping and variation map analysis of SARS-CoV-2

Figure 3. Genotyping and variation graph analysis using the μCaler SARS-CoV-2 Panel on SARS-CoV-2 samples collected through oropharyngeal swabs. The whole genome sequence of SARS-CoV-2 was assembled using IRMA software, and Nextclade was used for online analysis. Genotyping and variation map analysis was performed on the variant of Wuhan-Hu-1/2019 (MN908947) as the reference genome for alignment. A. Variation map of nucleotide sequence; B. Variation map of S protein amino acid variation.

For research use only. Not for use in diagnostic procedures.

Blocking

Color of tube cap

Component

Volume

1106102 (16 rxn)

Volume

1106101 (96 rxn)

μCaler NanoBlockers (for Illumina^®)

40 μL

210 μL

Color of Tube Cap

Component

Volume

1106212 (16 rxn)

Volume

1106211 (96 rxn)

μCalerNanoBlockers (for MGI, DI)

40 μL

210 μL

Hybridization Reagents

Packing No.	Color of Tube Cap	Component	Volume 1105102 (16 rxn)	Volume 1105101 (96 rxn)
Packing No.	Color of Tube Cap	Component	Volume 1105102 (16 rxn)	Volume 1105101 (96 rxn)
Box 1		μHyb #1	840 μL	3×1700 μL
		μHyb #2	130 μL	790 μL
		Human Cot DNA	45 μL	260 μL
Box 2		Streptavidin Beads	480 μL	2.9 mL
	/	Wash Buffer A	2×5.3 mL	3×21 mL
	/	Wash Buffer B	2.9 mL	20 mL

Capture Panel

Color of Tube Cap

Component

Volume

1101301 (96 rxn)

μCaler SARS-CoV-2 Panel

210 μL

Type	Product	Detail	Catalog#
Blocker	μCaler NanoBlockers (for Illumina®), 16 rxn	16 rxn	1106102
	μCaler NanoBlockers (for Illumina®), 96 rxn	96 rxn	1106101
	μCaler NanoBlockers (for MGI, DI), 16 rxn	16 rxn	1106212
	μCaler NanoBlockers (for MGI, DI), 96 rxn	96 rxn	1106211
Hybrid Capture	μCaler Hybrid Capture Reagents, 16 rxn	16 rxn	1105102
Hybrid Capture	μCaler Hybrid Capture Reagents, 96 rxn	96 rxn	1105101
Panel	μCaler SARS-CoV-2 Panel, 96 rxn	96 rxn	1101301